We infested three fish species, blenny (Salaria fluviatilis), Adriatic sturgeon (Acipenser naccarii) and gambusia (Gambusia holbrooki), with glochidia of the endangered freshwater mussel Margaritifera auricularia at different holding temperatures, and developed a system to grow and maintain cultured juvenile mussels. The sturgeon and blenny were successfully infested, although these fish sloughed the glochidia in the 24°C experiment. Release of juveniles began 30 days post-infestation for sturgeon and 31 days post-infestation for blennies, and excystment occurred over 8 and 10 days, respectively. The total number of juveniles cultured was 2,562. Although the mortality rate was high, juveniles were maintained alive for at least 4 weeks in the four test tanks. In the most successful tank, 13 juveniles were found after 6 weeks (39 days) of culture. Maximum dimensions for juveniles after culture were: length= 325 pm, width= 250 pm, height = 500 pm, indicating a mean increase of 51% in length, 60% in height and 23% in width.
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