Newly studied material of A. Falniowski’s collection of Bithyniidae from Greece and samples collected by V. Peic revealed three new species: Pseudobithynia ambrakis n. sp., P. euboeensis n. sp., and P. zogari n. sp., and in addition we could redescribe Bithynia hellenica Kobelt 1892. In total, 16 taxa of the Bithyniidae are now known from Greece, and the species here described gave us new insight as to their distribution pattern.